Interfacial and emulsifying properties of the electrostatic complex of β-lactoglobulin fibril and gum Arabic (Acacia Seyal)
Gao, Zhiming, Huang, Ying, Hu, Bing, Zhang, Ke, Xiaofei, Xu, Fang, Yapeng, Nishinari, Katsuyoshi, Phillips, Glyn O and Yang, Jixin (2018) Interfacial and emulsifying properties of the electrostatic complex of β-lactoglobulin fibril and gum Arabic (Acacia Seyal). Colloids and Surfaces A: Physicochemical and Engineering Aspects, 562. pp. 1-7. ISSN 0927-7757
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GURO_375_2018 --- Colloids and Surfaces A Accepted Manuscript.pdf - Accepted Version Download (1MB) | Preview |
Abstract
Formation, interfacial and emulsifying properties of the electrostatic complex of β-lactoglobulin fibril (BLGF) and gum Arabic Acacia Seyal (AS) were investigated. Necklace-like soluble complex could be formed at pH 3.5, and its charge and interfacial properties depended on the BLGF content. With appropriate amount of BLGF (< 9.09 wt.%), the formed complex possessed a good dispersibility and surface activity. When excessive BLGF (9.09∼50 wt.%) existed, surface charge of the complex was gradually neutralized and aggregation occurred. Homogeneous oil-in-water emulsions could be stabilized by the complex and the droplet size decreased with increasing BLGF content. Higher content of BLGF (9.09∼50 wt.%) was detrimental for emulsification due to the aggregation of complex, and the formed emulsion tended to flocculate. Compared with AS, the complex formed emulsions were much more stable against heating (90 ℃, 30 min) and salting (200 mM NaCl) environments, and the emulsions were stable during long-term storage (46 days). Proposed mechanisms for the adsorption of BLGF/AS complex at the oil-water interface. Pure AS (i) could adsorb at the oil-water interface but formed a loose film due to its poor surface activity and insufficient adsorption amount. With addition of a small amount of fibrils (ii), soluble electrostatic complexes are formed and they can be adsorbed at the interface to formed a dense viscoelastic film due to the surface activity of the BLGF. With a higher content of fibrils (iii), surface charge of the complex tended to be neutralized, causing the aggregation. Because the presence of protein fibrils, they could also adsorb at the oil-water interface to produce a viscoelastic film. However, with a bigger size and irregular shape, the aggregates were difficult to array at the interface as densely as the soluble complex.
| Item Type: | Article |
|---|---|
| Divisions: | Applied Science, Computing and Engineering |
| Depositing User: | Hayley Dennis |
| Date Deposited: | 20 Nov 2018 11:30 |
| Last Modified: | 05 Nov 2019 04:40 |
| URI: | https://wrexham.repository.guildhe.ac.uk/id/eprint/17353 |
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